Includes bibliographical references.
|Statement||by John R. Sargent.|
|The Physical Object|
|Pagination||107 p. :|
|Number of Pages||107|
Methods in zone electrophoresis. Poole, British Drug House Chemicals Ltd., (OCoLC) Online version: Sargent, John R. Methods in zone electrophoresis. Poole, British Drug House Chemicals Ltd., (OCoLC) Document Type: Book: All Authors / Contributors: John R Sargent. Using the different HPCE methods (zone electrophoresis in free solutions or in sieving/gel media, isotachophoresis, isoelectric focusing, affinity electrophoresis, and electrokinetic chromatography), the following physicochemical characteristics of a wide spectrum of compounds can be determined: effective, ionic and limiting mobilities. Summary This chapter contains sections titled: Introduction Continuous Zone Electrophoresis Simple Filter Paper Electrophoresis Simple Zone Electrophoresis for Preparative Purposes Column Electroph. This book is designed to help you make the most of your CE separations, and includes comprehensive information on: Electroosmosis, separation efficiency, and Joule heating Detection methods In-depth discussion of the separation principles and capabilities of the major modes of CE Sieving gel electrophoresis Isoelectric focusing Free solution CE.
Electrophoresis terminology There are a few significant differences between the nomenclature of chromatography and capillary electrophoresis. For example, a fundamental term in chromatography is retention time. In electrophoresis, under ideal conditions, nothing is retained, so the analogous term becomes migration time. Capillary electrophoresis (CE) has, over the years, demonstrated its powerful separation ability in the area of chiral and achiral analysis. This is contributed to the advantages it offers when compared to chromatographic techniques: (1) low consumption of samples and solvents; (2) high separation efficiency and resolution; (3) versatility [ 1 Cited by: 1. Additional Physical Format: Online version: Sargent, J.R. (John R.). Methods in zone electrophoresis. Poole, Eng., British Drug Houses, B.D.H. Laboratory Chemicals. This book is divided into 14 chapters and begins with a brief introduction to the general principles of zone electrophoresis. The subsequent chapters deal with the principles, instrumentation, and applications of various methods of zone electrophoresis, including low- and high-voltage paper, cellulose-acetate, thin-layer, agar- and starch-gel Book Edition: 2.
In Capillary Electrophoresis of Carbohydrates, hands-on experts describe cutting-edge techniques in capillary electrophoresis (CE) for the analysis of complex carbohydrates. Written in step-by-step detail to ensure successful experimental results, these readily reproducible protocols provide methods for sample preparation and analysis of mono Author: Pierre Thibault. Capillary Zone Electrophoresis (CZE) Capillary Zone Electrophoresis (CZE), also known as free solution capillary electrophoresis, it is the most commonly used technique of the six methods.A mixture in a solution can be separated into its individual components quickly and separation is based on the differences in electrophoretic. In zone electrophoresis, the U-tube was replaced by paper, a support material employed simply to prevent or minimize diffusion of ions, so that ions applied in a narrow strip to the paper will separate and remain as relatively discrete : Stephen M. Palfrey. In this article we will discuss about Electrophoresis: 1. Meaning of Electrophoresis 2. Definition of Electrophoresis 3. Classification. Meaning of Electrophoresis: The term electrophoresis describes the migration of a charged particle under the influence of electric field (electro-charged particle and phoresis-movement). Many important biological molecules such as amino acids, peptides.